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Master Tezleri

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Browsing Master Tezleri by Author "Acar, Ertuğrul"

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    Master Thesis
    Assesment of genes that play role in phenolic compound degradation in olive orchard microbiome
    (Izmir Institute of Technology, 2019-10) Acar, Ertuğrul; Soyer Dönmez, Ferda
    The olive tree (Olea Europea L.) is one of the most important fruit trees in Mediterranean countries. Its products, olive oil and table olives, are important components of the Mediterranean diet and widely consumed all around the World. Olives and virgin oil provide a rich source for phenolic compounds. The plant phenolics are secondary metabolites, and possesses several common biological and chemical properties. In this study, microorganisms were collected from soil, olive leaves, fruits, and Olive fruit fly larva and Olive mill wastewater (OMWW). They were characterized by 16S rRNA analysis. The microorganisms that were tolerant to phenolic compounds were selected in order to seek which genes were associated with the phenolic compound degradation. The genes related to the degradation of the selected organisms were identified by Sanger Sequencing and the level of phenol-degrading gene expression were aimed to be compared by using qPCR and Droplet Digital PCR (ddPCR). Microorganisms which degrade phenolic compounds can be harnessed for the purpose of bioremediation. However, the number of defined phenolic compound degrading microorganisms is still low in the literature. For this reason, many different microorganisms were used at the same time for bioremediation. Investigation of olive orchard microorganisms and phenolic-degrading genes might benefit bioremediation in the future. In this study, 8 different bacterial strains were identified and characterized from olive orchards. After that, their phenol hydroxylase and catechol 1,2 dioxygenase genes tried to be sequenced with primers designed by using of reference strains in NCBI database.