Browsing by Author "Mete, Derya"

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Investigation of the mechanism of action of 6-bicycloaryl substituyed 5,6-dihydro-2H--pyran-2-ones
(Izmir Institute of Technology, 2013) Mete, Derya; Çağır, Ali
In cancer research, naturally isolated 5-substituted, Î±,Î²-unsaturated lactones have become attracted the attention of scientists increasingly due to its biological activities. One of the most important member of this group of compounds is goniothalamin. Goniothalamin has high cytotoxic activity against various cancer cells and causes a minimal effect on healthy cells so it is believed that it has selective cytotoxic activity against cancer cells. In the first part of this study, anticancer activities of novel 5,6-dihydro pyran derivatives were determined by MTT assay in MCF7, PC3, HCT116 p53+/+ and p53-/- cell lines. Apoptotic effects of the selected compounds were also examined with the help of FITC labeled Annexin V-PI in flow cytometry . In the second part, anti-cancer compounds having high cytotoxic compounds were chosen and tested for their effect on cell cycle and apoptosis mechanism by flow cytometry and western blot analysis.
Rhodamine-Immobilised Electrospun Chitosan Nanofibrous Material as a Fluorescence Turn-On Hg²⁺ Sensor
(Wiley-v C H verlag Gmbh, 2016) Horzum, Nesrin; Mete, Derya; Karakus, Erman; Ucuncu, Muhammed; Emrullahoglu, Mustafa; Demir, Mustafa M.
A turn-on fluorescence sensing system for mercuric (Hg2+) ions relying on a modified rhodamine B-chitosan fluorophore moiety was developed. This novel sensing approach relies on the simultaneous electrospinning of chitosan and rhodamine B hydrazide with phenylisothiocyanate functionality in hexafluoroisopropanol solution at 3.4 kV cm(-1). The electrospun mats exhibited not only considerably enhanced fluorescence intensity in the presence of mercury ions, a result attributed to the ring opening of the spirolactam unit of the rhodamine-based fluorophore, but also a remarkably high sensitivity and selectivity toward Hg2+. In effect, the strategy has the potential to open new avenues in the design and development of other high-performance nanofibrous sensing materials for detecting target metal species of environmental interest.
Synthesis, characterization and investigation of cytotoxic effects of drug loaded ZIF-8 metal-organic frameworks
(Izmir Institute of Technology, 2021-12) Mete, Derya; Şanlı Mohamed, Gülşah
The biocompatible ZIF-8 intelligent material, a member of the metal-organic framework family, has a biodegradable property in an acidic environment due to its poor coordination bonds. Because cancerous cells are more acidic than healthy cells, our studies aim to ensure that doxorubicin, sorafenib, and apalutamide, encapsulated in ZIF-8, target cancer cells responsive to pH, thereby reducing damage to healthy cells. In addition, ZIF-8 was selected not only as a carrier system but also as a therapeutic effect. Because ZIF-8 material is biodegradable, it is divided into zinc and 2-methylimidazole components in cancer cells. Research shows that the decrease in the amount of zinc is essential in the formation of cancer cells. Zinc is reported to be in lower intracellular concentrations in HCC and prostate cell lines instead of healthy variants. It aims to create dual cytotoxic effects on cancer cells by combining the effects of zinc-drug on a single platform.