Browsing by Author "Uslu, Mehmet Emin"

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Determination of apoptotic effects of clinoptilolite on human T lymphocytes
(Izmir Institute of Technology, 2008) Uslu, Mehmet Emin; Nalbant Aldanmaz, Ayten
Zeolites are defined as aluminum silicates that have made up of oxygen, aluminum and silicon. SiO4 and Alo4 tetrahedrals are the smallest units that give the specific shape to the molecule. There are more than 40 types of natural and over 150 types of synthetic zeolites occurs and those zeolites are used in agriculture, animal husbandry, architecture, pharmaceuticals and metallurgy. Recent years in literature it was shown that these zeolites can have regulatory effects on immune system. Also it was shown that they can influence the development of cancer and have a role on the expression of tumor suppressor genes. However there is no evidence can be found on how these molecules affect the function of specific cell types in the molecular level and also the mechanism of this effect.In this study the apoptotic effects of clinoptilolite which is a natural zeolite found in Gördes region of Turkey, on human T lymphocytes were studied. T cells were chosen in this study because they are the main players in the immune system. These cells can establish immune regulation and organize immune response. As a result, they are important in pathology and therapy. Peripheral blood mono nuclear cells (PBMCs) in which T cells can be found were isolated from the healthy donors blood by Ficoll Hypaque Gradient Method and then these cells were incubated with clinoptilolite in the RPMI 1640 media. Apoptosis were measured in FacsArray after appropriate immunofluorescent labeling and by agarose electrophoresis technique after DNA fragmentation assay done.
Investigation of hemostatic biomaterials containing plant extracts
(Izmir Institute of Technology, 2014) Uslu, Mehmet Emin; Bayraktar, Oğuz; Başal Bayraktar, Güldemet; Akhisaroğlu, Mustafa
Haemostasis in other words a process which causes bleeding to stop is very important in injury. Recent researches were focused on discovery of haemostatic agents and developing biomaterials which transfer them to the injury side. Therefore plant extracts and three dimensional biomaterials were widely investigated. In this research the extract of Equisetum arvense was investigated as a coagulatory agent. Silk fibroin-hyaluronic acid mixture was used for the preparation of three dimensional sponge like biomaterials. The prepared sponge forms could also be used as scaffold for wound healing. In this research effect of extraction parameters on extract composition and bioactivity was investigated. Effect of extraction parameters were analyzed on 6 factors at 3 levels. It was seen that extraction parameters had high influence on both composition and bioactivity of the prepared extract. Although it was reported that the extract of equisetum arvense had anticoagulant activity, in this research it was shown that changing parameters caused variation of extract bioactivity from anticoagulant to coagulant as a result of changing extract composition. Addition of extract into silk fibroin-hyaluronic acid mixture caused proteins to precipitate as a result of interaction between protein and phenolic compounds. As a result of this precipitation significant decrease in the mechanical strength of biomaterial was observed. In order to minimize this interaction, plant extract was added into the silk fibroin after mixing with hyaluronic acid solution. As a result, mechanical strength and pore size of the biomaterial were increased and pore distribution became more regular. Also biomaterial gained tubular network on both vertical and horizontal dimensions. This would help the proliferation and migration of the fibroblast cells and moreover prevent the formation of scar tissue.