Master Tezleri
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Browsing Master Tezleri by Department "Molecular Biology and Genetics"
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Master Thesis 3D reconstruction using a spherical spiral scan camera(Izmir Institute of Technology, 2006) Vatansever, Mustafa; Aytaç, İsmail SıtkıConstruction of 3D models representing industrial products/objects is commonly used as a preliminary step of production process. These models are represented by a set of points which can be combined by planar patches (i.e. triangulation) or by smooth surface approximtions. In some cases, we may need to construct the 3D models of real objects. This problem is known as the 3D reconstruction problem which is one of the most important problems in the field of computer vision. In this thesis, a sytem has been developed to transform images of real objects into their 3D models automatically. The system consists of a PC, an inexpensive camera and an electromechanical component. The camera attached to this component moves around the object over a spiral trajectory and observes it from different view angles. At the same time, feature points of object surface are tracked using a tracking algorithm over object images. Then tracked points are reconstructed in 3D space using a stereo vision technique. A surface approximation is fitted to this 3D point set as a last step in the process. Open source .Intel®-OpenCV. C library is used both in image capturing and in image processing.Master Thesis Analysis of lysosomal Neu4 sialidase associated proteins by using mass spectrometry (MS/MS)(Izmir Institute of Technology, 2012) Öztürk, Süleyman Can; Seyrantepe, VolkanSialidases are glycohydrolytic enzymes which remove sialic acid residues from glycoproteins, oligosaccharides and glycolipids. There are 4 different sialidases known in mammalians. These are Neu1 (lysosomal), Neu2 (cytoplasmic), Neu3 (cell membrane) and Neu4 (lysosomal/mitochondrial) sialidase. Sialidases are involved in many metabolic and cellular processes interactioning with another proteins or work together in multiprotein complexes. For example, Neu1 is only active with betagalactosidase and cathepsin A enzyme in lysosome. Interactions of sialidases Neu2, Neu3 and Neu4 enzyme with other proteins remain unknown In our study, we aimed to identify proteins which have interaction with sialidase Neu4 as well as Neu1 by using mass spectrometry analysis to find new possible roles of sialidases. Our bait protein's cDNA was tagged with calmodulin binding protein as well as streptavidin binding protein. After transfection and expression of vectors to mammalian cells, proteins were purified using tandem affinity purification (TAP). We identified some associated proteins with sialidase Neu1 and Neu4 by using MS/MS analysis and bioinformatics.Master Thesis Analysis of temporal and spatial expression of drosophila embryonic small RNAs by deep-sequencing method(Izmir Institute of Technology, 2012) Coşacak, Mehmet İlyas; Akgül, BünyaminThe world of small RNAs is expanding and new types of small RNAs are being identified. By using deep-sequencing techniques in addition to most abundant small RNAs; miRNA and siRNA, piRNA and tRFs were further characterized and shown to be functional. The global behavior of small RNAs during MZT and their location in the cytoplasmic complexes has not been shown. By combining polysomal fractionation and deep-sequencing technique as well as the highly regulated developmental stages in Drosophila we have shown that the temporal and spatial expression of small RNAs changes during MZT. We have shown that each small RNA group has unique behaviour in cytoplasm and is enriched in specific polysomal fractions which shows that their local function in cytoplasmic complexes is mainly translational machinery.Master Thesis The assessment of a new relationship between from and structure in digital architectural design affter 1990's(Izmir Institute of Technology, 2013-07) Demir, Yelin; İnceköse, ÜlküFrom the beginning of 1960’s, architects are using computers in architectural design processes. Initially, architects used them as design tools for representation and modelling purposes. However from the beginning of 1990’s, architects started to use computers as design mediums where the whole design process evolves starting from design ideation to construction. Digital technologies enabled architects to design wide range of forms in digital design environments and therefore, form-based design processes appeared. Architects mostly focused on form generation concerns. However, this form based approach brought forth problems in relation to constructability. Complex irregular forms challenged engineers to find optimal structural solutions. Architects and engineers started to work on a unified architectural and structural design process and offered new structural system solutions with the help of digital technologies for overcoming the constructability problem. This process and solutions presented different form-structure relationships. The main aim of this thesis is to reveal the form-structure relationships in digital design processes focusing on constructability of digitally designed forms. Constructability is related to structural system design. Therefore, this thesis examines design, manufacturing and construction processes of structural systems to understand how architectural designs/forms are realized and constructed. It examines the whole process of design to construction of structural systems including models, tools, structural system solutions, and fabrication and construction methods. The examination is done over case studies which are digitally designed and constructed building examples. Each case study exemplifies a different model, tool, structural system and design process which also defines a different type of form-structure relationship. Key words: digital architectural design, structural design, form, structure, constructionMaster Thesis Assessment of Izmir Halkapınar Water Pump Station for its conservation as industrial heritage(Izmir Institute of Technology, 2019-06) Şener, İdil Ece; İpekoğlu, BaşakNew technologies have been used in water distribution as a result of the modernization brought about by the Industrial Revolution started in Europe in the 18th century. Ottoman Empire also benefited from the technologies when granted privileges to foreign companies to establish water supplies in the port cities. Among these companies, “La Compagnie Ottomane des Eaux de Smyrne” (Ottoman Water Company of İzmir) built Halkapınar Water Pump Station building in İzmir in 1898. The building, which still maintains its original function, is located within the facility of İzmir Water and Sewerage Administration (İZSU) in Halkapınar. It consists of the main pump building and two outbuildings The aim of this study is to analyse the values and problems of the structure and to develop a conservation proposal. The method applied in the study is field survey, archives and literature research. It is a rare example of surviving industrial heritage and has a significance in reflecting the technological developments in water distribution in the 19th century. The problems of the building are poor quality additions and removals implemented throughout the years. As a result of the study, it is proposed to maintain the original function of the building, to remove poor quality additions in the main pump station and on the original outbuilding, to rearrange the original features, and open to public visitation. The proposed interventions considering the values of the building will contribute to the conservation and exhibition of the building as an industrial heritage.Master Thesis Assessment of liposomal formulations and biological activities of eggplant glycoalkaloids(Izmir Institute of Technology, 2019-07) Tatlıdil, Engin; Frary, AnneBillions of dollars are spent every year in the world for cancer treatments and research. In recent years, bioactive compounds are being tested as promising therapeutics. Among these compounds, eggplant glycoalkaloids: solasonine and solamargine are known to be effective against skin cancer and diseases. However, these compounds are water insoluble. This reduces transdermal drug delivery and the efficacy of solasonine and solamargine. Nanocarriers are used for transdermal drug delivery of water insoluble molecules. In this study, liposomes were used as nanocarriers to increase drug delivery of solasonine and solamargine. In the first stage of the study, empty liposomes produced from four different lecithin types with hydration temperatures of 40°C, 45°C and 50°C were evaluated according to their physical, chemical stability and drug loading capacity criteria at three different storage temperatures (4°C, 25°C, 37°C). The liposome formulation which was most suitable for the continuation of the study was determined. In the second stage of the study, solasonine and solamargine loaded liposomes were produced according to the formulation determined in the first stage and these liposomes were evaluated according to their physical, chemical stability, zeta potentials and drug leakage rate criteria for 3 months and it was determined that the drug loaded formulation was stable during the monitoring process. Furthermore, the release profiles of the drugs in different release media were determined and also the efficacy of the free and encapsulated states of solasonine and solamargine were tested in HaCaT and SCC-25 cell lines and IC50 values were determined.Master Thesis Association mapping for oil and protein content in hazelnut (Corylus avellana L.)(Izmir Institute of Technology, 2020-06) Söylemez, Fatmanur; Doğanlar, SamiEuropean hazelnut has an important place in terms of nutritional and economic value among tree nut species. Because of its nutritional content, the consumption of hazelnuts promotes human health in many ways. These nutritional components are controlled by multiple genes and affected by the environment; therefore, they are quantitative traits. The vast majority of world hazelnut production is provided by Turkey. So it is important to develop hazelnuts with higher nutritional quality for our country. In this work, we aimed to associate genetic diversity data and oil and protein content of hazelnuts to identify QTL. For this purpose oil and protein content were measured in kernels of 96 accessions. Genotypic data were obtained with 30 SSR markers and resulted in 407 polymorphic alleles. According to allelic data, the mean dissimilarity value was 0.52 (52%) for the 96 accessions. Population structure analysis resulted in three clusters with 30, 30, and 16 accessions. Twenty accessions could not be assigned to any cluster and were considered admixed. Association mapping between allelic and phenotypic data indicated that five loci were significantly associated with oil content. The most significant result for oil content belonged to B628-307 loci (p=0.0002, r2=0.145). Three loci were detected for protein content. Among them, A613-153 had the most significant effect (p=0.003, r2= 0.088). We hope that our survey of germplasm and the identified loci associated with oil and protein amount can accelerate hazelnut breeding. In the future this study can contribute to develop new genotypes with enhanced nutritional value.Master Thesis A bibliometric analysis of critical construction management studies(Izmir Institute of Technology, 2019-07) Deniz, Berna Derya; Kale, SerdarCopernicus's revolution is a philosophical change that questions the existence of humanity in the universe, as opposed to the belief; he claims that the Sun is the center of the universe, not the Earth. Rather than questioning a different perspective, believing in a dogmatic knowledge is a simple way to escape. Critical Management Studies (CMS), like Copernicus's revolution, has an ambitious goal on management theory and practice. CMS offers a number of alternatives to the current system by expressing more than critical thinking. It focuses on the impacts of management on the traditional labor force and production factors and it focuses on organizing the differences between theory and practice. The aim of the research presented in this thesis is to explore the evolution of CMS and its diffusion in project management studies and construction management studies. Bibliometric analysis is chosen as the research method, in the citation networks that emerged using the VOSviewer program, 11 most cited articles from the 283 center documents underlie the basis of the study. In the study, the citation relations of the articles are examined in terms of author, document, country, and keyword. The results of the research indicate that (1) CMS is still in the process of embryotic stage of its development, (2) the diffusion of CMS in project management studies and construction management literature have been limited and (3) the calls for pluralistic approach in construction management studies have not been addressed in literature.Master Thesis Biochemical and molecular characterization of extracellular enzyme producing Staphylococci isolated from different origins(Izmir Institute of Technology, 2006) Appak, Sıla; Güneş, HaticeStaphylococci are pathogenic bacteria known to cause diseases among diferrent organisms including human. The two species Staphylococcus aureus and Staphylococcus epidermidis are well defined in human diseases although their exact mechanism of pathogenesis is still not fully understood. These pathogenic bacteria could be isolated from soil, water, air, as well as from the living organisms and they are both pathogenic and saprophytic.Extracellular enzymes of the organisms are used for the industrial purposes. The isolation and characterization of these enzymes are crucial steps in biotechnology. The extracellular enzymes derived from the bacteria serve for many purposes in the industry. In this project 128 Staphylococcus sp. were used. Of these 128 bacteria, 12 were isolated from patients, 40 were isolated from the foodhandler.s hygiene detections, 27 were isolated from pygeons and 49 of them were reference strains. They were searched for the presence of some of the industrially important extracellular enzymes: protease, lipase, cellulase, xylanase, amylase, laccase, urease, DNase and pectinase with biochemical tests. They were also searched for the presence of the lipase, protease and thermonuclease amplifications by PCR. The bacteria apart from the refence strains were also tried to be identified by 16S-ITS-rRNA RFLP analysis. The results would indicate the extracellular enzyme production among these pathogenic bacteria and would also be used as a guide in further studies to correlate between Staphylococcal pathogenity and enzyme production.Master Thesis Bioinformatic approaches to investigate HIV capsid-nanobody interaction(01. Izmir Institute of Technology, 2023-07) Atik, Şeref Berk; Taşkent Sezgin, HümeyraInfection with HIV is still a global pandemic. Since the discovery of this highly mutagenic virus, nearly 40 million people have passed away as a result of HIV-related health problems. Currently, 38.4 million people are HIV-positive. Following infection, the viral genome gets integrated into the host cell genome. The infected person carries the virus for the rest of their life and can spread it to others through bodily fluids. Because there is no treatment for HIV, the World Health Organization recommends that infected people be diagnosed early through comprehensive screening to restrict the virus's spread. As a result, there is still a need to create practical, sensitive diagnostic tools, particularly for use in the field of HIV infection testing. In this study, the interaction between HIV-1 capsid protein, the first antigen found in the blood during the acute phase of HIV infection, and a nanobody (Nb, a single domain antibody) known to bind to capsid is investigated at the molecular level through computational methods. Because the structure of HIV-1 CA binding-Nb is unknown, all-atom models of the Nb structure were constructed using comparative methods, deep-learning-based methods, and hybrid methods (SwissModel, trRosetta, Robetta, AlphaFold2), and promising models were chosen. In the second stage, molecular docking was used to produce HIV-1 capsid- nanobody complex structures, which were then tested for stability and native-likeness using standard molecular dynamics simulations. Understanding the molecular details of the HIV-1 capsid-nanobody complex, we believe, will provide essential data for using this antigen-antibody pair inan immunosensor system for HIV-1 infection diagnosis.Master Thesis Cell adhesion on nanomater scale fibronectin patterns: A comparision of breast cancer cells and normal breast epithelial cells(Izmir Institute of Technology, 2014-12) Horzum, Utku; Pesen Okvur, DevrimCell adhesion to extracellular matrix is an important process for both health and disease states. Surface protein patterns are topographically flat, and do not introduce other chemical, topographical or rigidity related functionality and, more importantly, that mimic the organization of the in vivo extracellular matrix are desirable. Previous work showed that vinculin and cytoskeletal organization are modulated by the size and shape of surface nanopatterns. However, a comparative and quantitative analysis on normal and cancerous cell morphology and focal adhesions as a function of micrometer scale spacings of protein nanopatterns was absent. Here, electron beam lithography was used to pattern fibronectin (FN) nanodots with micrometer scale spacings on a K-casein background (single active) on indium tin oxide (ITO) coated glass which, unlike silicon, is transparent and thus suitable for many light microscopy techniques. Exposure times were significantly reduced using the line exposure mode with micrometer scale step sizes. Micrometer scale spacings of 2, 4, 8 microns and gradients between FN nanodots modulated cell adhesion for both breast cancer and normal mammary epithelial cells, through modification of cell area, cell symmetry, actin organization, focal adhesion number, size and circularity under both static and flow conditions. Overall, cell behavior was shown to shift at the apparent threshold of 4 μm spacing. Results showed that there were significant differences in terms of cell adhesion between breast cancer and normal mammary epithelial cells: Breast cancer cells exhibited a more dynamic and flexible adhesion profile than normal mammary epithelial cells.Master Thesis Characterization and genetic mapping of health related traits in tomato(İzmir Institute of Technology, 2005) Rusçuklu, Dane; Doğanlar, SamiTomato is consumed in the daily diet and has a diverse nutritional content including antioxidants, lycopene, vitamin C and phenolics. However, the content of these compounds in tomato cultivars could be higher. There are two ways of increasing the nutritional quality of tomato. One of them is a transgenic way which is still questioned and is not approved in many countries. The other way is molecular breeding which is based on introgression of wild genes into cultivated tomatoes via the combination of classical breeding with molecular marker analysis. This method lets us obtain nontransgenic tomatoes with increased nutritional quality. In this project a population that was obtained from a cross between wild type L.pimpinellifolium and cultivated tomato L.esculentum was used. This population includes 145 inbred backcross lines (IBLs). It was expected that some individuals of this population had increased nutritional quality derived from the wild parent. In order to test this hypothesis, antioxidant activity of each line was measured. The method used to measure antioxidant activity was based on spectrophotometric measurement which gave mol Trolox equivalent antioxidant activity. It was observed that the IBLs were phenotypically close to L.esculentum and that some individuals did have higher antioxidant activity than the cultivated parent. The population was also mapped with molecular markers and 31 possible QTLs which control antioxidants in tomato were found. The source of alleles associated with increased antioxidant characters was usually L.pimpinellifolium. This result confirmed that wild type tomatoes can be a resource for increasing nutritional quality of tomatoes.Master Thesis Characterization of genes that play role in manganese tolerance in different yeast species(01. Izmir Institute of Technology, 2021-07) Karagöz, Ezgi; Karakaya, Hüseyin Çağlar; 01. Izmir Institute of TechnologyManganese is an essential element for organisms that can also be toxic. It has been stated that more than 5 mM Mn inhibits the growth of wild-type S. cerevisiae BY4741 strain. In the cases when this amount is exceeded, the stress upon manganese toxicity arises and it leads to a range of responses to normalize the manganese level. However, the genes accountable for that case are unknown. Manganese toxicity is a restrictor factor in the production of agricultural products. Identification and characterization of the genes that play a role in manganese homeostasis are rather essential. In this study, we have used Sanger Centre's Saccharomyces Genome Resequencing Project (SGRP) strains, which are collected from different regions of the world. After screened the whole collection, we have identified four manganese resistant strains; S. cerevisiae BY474, S. paradoxus Y6.5, S. cerevisiae 378604X and S. paradoxus Q74.4. Manganese-related genes were selected via the Saccharomyces genome database (SGD). Expression levels of these genes under manganese stress in most resistant strain Q74.4 analyzed by RT-Q-PCR. As a result, GCR-1 dependent translation factor GDT1 and high-affinity phosphate transporter PHO84 were found to be upregulated in Q74.4 that endure high levels of manganese toxicity. These genes are probably accountable for manganese tolerance in Q74.4 strain. The results arising from that study, will take the lead to the development of biotechnological exercises for manganese bioremediation. Meanwhile, it might help molecular mechanisms to be able to develop resistance to stressful conditions that manganese generates and shed light to further studies.Master Thesis Characterization of glycoalkaloid content and molecular mapping in eggplant(Izmir Institute of Technology, 2006) Kırsoy, Öyküm; Frary, AnneIn this thesis, solamargine which is a known eggplant glycoalkaloid and has an important place for human health was characterized in eggplant. For characterization, two eggplant lines S.melongena MM738 and S.linnaeanum MM195 were used. Although, for identification and detection of glycoalkaloid concentration, many different methods have been utilized, for this thesis, high-performance liquid chromatography (HPLC) was used to analyze glycoalkaloid concentration in eggplant. In HPLC, spiking of samples was done using a solamargine standard and it was found that S. melongena had an undetectable level of solamargine while S. linnaeanum had between 17.6 and 33.4 mg (average 25.5 ± 11) solamargine per gram of freeze dried powder. In addition to characterization of glycoalkaloids in S.melongena MM738 and S.linnaeanum MM195, different types of molecular markers were surveyed for polymorphism in S.melongena MM738 and S.linnaeanum MM195 for mapping. A total of 47 polymorphic markers were then tested on the F2 population and located on the eggplant molecular genetic map.Master Thesis Characterization of outer membrane proteins of Salmonella Enteritidis in response to phenolic acids stress(Izmir Institute of Technology, 2012) Özdemir, Özgün Öykü; Soyer Dönmez, FerdaSalmonella enterica serovar Enteritidis (Salmonella Enteritidis) is one of the most reported foodborne pathogen bacterium throughout the world that causes large outbreaks and may result in deaths. Phenolic acids, synthesized in almost all plants, are important for plant metabolism and protection of plants. They can be thought as promising antimicrobial agents against Salmonella Enteritidis. Outer membrane proteins are unique structures to Gram-negative bacteria including Salmonella genus and they are the first targets to environmental changes. This study showed the antimicrobial effect of 3-HPAA, cinnamic acid and o-coumaric acid on Salmonella Enteritidis in vitro. Scanning electron microscopy was used for showing the changes in bacterial shapes. Their effects on the outer membrane protein profile were investigated via proteomic approach. According to results of 96-well microtiter plate assay used for antimicrobial effect determination, among these phenolic acids, 3-HPAA showed 100% growth inhibition and other phenolics retarded the bacterial growth. 30 mM was found as minimum inhibitory concentration (MIC) of 3-HPAA at 24th hour of the experiment. SEM results demonstrated that 3-HPAA causes bacteria to have collapses on cell surface while cinnamic acid and o-coumaric acid cause cells to elongate abnormally. SDS PAGE of the OMPs of bacteria who were exposed to phenolic acids showed changes in amounts of proteins in some protein bands compared to control OMPs. 2-D PAGE results of OMPs of control bacteria via OMPs of 3-HPAA, cinnamic acid and o-coumaric acid treated bacteria, changes in sizes of spots were observed.Master Thesis Characterization of pollen-E1 gene might play role in salt tolerance in Beta maritima(Izmir Institute of Technology, 2015-07) Uysal, Özge; Karakaya, Hüseyin ÇağlarSalinity stress has a negative impact on the growth of plants, which affects homeostasis and productivity. The uptake of non-essential salt ions change the osmotic balance of the cell and cause dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. In this project, we isolated and characterized salt tolerance genes in Beta maritima plant. For this purpose, functional genomics technique was used by over expressing cDNAs in yeast and colonies can grow toxic salt media isolated and characterized. We found several colonies and we focused on uncharacterized Pollen-E1 gene with an unknown function. Pollen-E1 cDNA confers salt tolerance to yeast cells. Intracellular sodium measurements of Pollen-E1 overexpressed in yeast cells showed decreased salt levels as compared to wild type suggesting that sodium was transported out of the cell. Pollen-E1 protein localized in endomembrane systems in the yeast cells. In mRNA expression analysis, Pollen-E1 mRNA levels induced immediately in leaves and later stages in root systems under salt stress. Our results showed that is the uncharacterized and unknown function Pollen-E1 gene might have some role of regulating salt tolerance in Beta maritima.Master Thesis Cloning of polysome-associated small RNAs in Drosophila melanogaster embryos(Izmir Institute of Technology, 2009) Yiğit, Hatice; Akgül, BünyaminGenome-encoded regulatory small RNAs are classified into 3 groups; microRNAs (miRNAs), endogeneous small interfering RNAs (endo siRNAs) and piwi interacting RNAs (piRNAs). miRNAs, 17-21 nucleotide in size, are involved in posttranscriptional gene regulation via precise or imprecise base pairing with target mRNAs resulting in either target mRNA degradation or translational inhibition. Endo siRNAs ,on the other hand, may function transposon regulation but their precise regulatory function and mechanism have not been elucidated yet. piRNAs are mainly involved in transposon silencing in spermatogenesis. Despite their discovery, biological roles and modes of functions of small RNAs remain to be elucidated. The aim of this thesis was to identify polysome-associated small RNAs in Drosophila melanogaster embryos by deep sequencing and investigate their role in translational regulation. Deep sequencing and microarray results determined stage and fraction specific distribution of genome encoded small RNAs. Surprisingly, the results implied that mRNAs may be posttranscriptionally regulated by antisense transcripts in polysome.Master Thesis A cluster based communication architecture for distributed applications in mobile ad hoc networks(Izmir Institute of Technology, 2006) Dağdeviren, Orhan; Erciyes, KayhanIn this thesis, we aim to design and implement three protocols on a hierarchical architecture to solve the balanced clustering, backbone formation and distributed mutual exclusion problems for mobile ad hoc network(MANET)s. Our ¯rst goal is to cluster the MANET into balanced partitions. Clustering is a widely used approach to ease implemen-tation of various problems such as routing and resource management in MANETs. We propose the Merging Clustering Algorithm(MCA) for clustering in MANETs that merges clusters to form higher level of clusters by increasing their levels. Secondly, we aim to con-struct a directed ring topology across clusterheads which were selected by MCA. Lastly, we implement the distributed mutual exclusion algorithm based on Ricart-Agrawala algo-rithm for MANETs(Mobile RA). Each cluster is represented by a coordinator node on the ring which implements distributed mutual exclusion algorithm on behalf of any member in the cluster it represents. We show the operations of the algorithms, analyze their time and message complexities and provide results in the simulation environment of ns2.Master Thesis Collaboration in architectural design competitions(İzmir Institute of Technology, 2013-07) Erbil, Livanur; Doğan, FehmiThis study investigates design collaboration with reference to convergent and divergent idea generation processes, conceptual, spatial, temporal and technological barriers, and roles and relationships in architectural design teams entering a design competition. Study of design teams offer a unique opportunity to investigate how creativity is fostered through collaborative work. While views of creativity often relate creativity to individual originality, collaboration requires different designers to work together towards one common design idea and consider as many different ideas as possible. In collaborative design, it would be easier to offer a variety of ideas but equally difficult to establish a consensus on a single idea. To investigate the role of convergent and divergent thinking in the design process, first, 30 professional architects, who participated in competitions as a team, were interviewed via phone. Phone interviews were analyzed thematically to investigate how teams came together and how team participants overcame spatial, temporal, conceptual, and technological barriers. Convergent and divergent idea generation phases were analyzed to understand how team participants generate multiple ideas and converge on one single issue. Second, three groups of architecture students, who participated in competitions as a team, were interviewed. Interviews were analyzed thematically to investigate how teams overcame spatial, temporal, conceptual, and technological barriers. It is concluded that barriers and roles of participants in design collaboration interact with convergent and divergent concept generation. Keywords: Collaboration; Architecture Competitions; Design Process; Design CognitionMaster Thesis Comparative analysis of master of industrial design education in Turkey(Izmir Institute of Technology, 2008) İmamoğulları, Beril; Erkarslan, ÖnderThis thesis focused on the master.s program in industrial design, which is research and practice oriented in the light of current themes and design principals. It argued that a master.s degree in industrial design would help graduates specialize in the related field and improve their skills. Therefore, this study consists of fundamental components about master.s studies in industrial design in turkey such as, existing circumstances of master.s degree, defining its problems and requirements, and resolution advisories to education.Seven universities offer a master.s degree in the field of industrial design in Turkey. In the study, comparative analysis of these programs is conducted on their current education system and two survey studies are realized among the academics of these universities, to examine their opinions.In the field study, the increase number of department facilities was underlined as one of the positive developments whereas problems concerning the academic staff of master.s programs came into prominence as the most important weaknesses. In addition, the required improvements of YOK criteria for the discipline were also found a critical situation for the future of programs.Keywords: Industrial Design Education, Master.s Degree Program, Comparative Analysis of Education.