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Regulation of human p53 tumor suppressor gene activity by thiol-depentdet oxidoreductases

dc.contributor.advisor Koç, Ahmet en
dc.contributor.author Bekki, Gözde
dc.date.accessioned 2023-11-13T09:49:43Z
dc.date.available 2023-11-13T09:49:43Z
dc.date.issued 2011 en
dc.description Thesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2011 en
dc.description Includes bibliographical references (leaves: 30-34) en
dc.description Text in English; Abstract: Turkish and English en
dc.description vii, 37 leaves en
dc.description.abstract Background: Occurrence of p53 mutations in more than half of human tumors indicates the importance of p53 gene in cancer prevention. Nevertheless, oxidation of cysteine-SH groups in p53 protein can inactivate the protein under oxidative conditions. The importance of p53 as a tumor suppressor and insufficient studies about redox regulation of p53 gene lead us to study redox regulation of p53 protein. Methods: In this study, yeast (Saccharomyces cerevisiae) was used as an in vivo model. All potential thiol-dependent antioxidant genes in yeast were identified based on specific characters in their structure, via REDOXCysSearch bioinformatics tool. To study human p53 gene activity in yeast cells, p53 gene and p53 RE Lac-Z reporter that is inducible by p53, were transformed into yeast. Antioxidant gene mutants were analyzed for LacZ reporter gene activity. Results: We identified several yeast mutants (Δsac1, Δhnt3 and Δmap1) with lower p53 activity with respect to wild-type yeast. Conclusion: Due to conserved mechanisms of cell cycle regulation and oxidative stress tolerance between yeast and mammals, we believe that results from yeast studies may help us to understand the redox regulation of p53 in human cells. Thus, a new perspective may appear in the redox regulation of p53 gene. en
dc.identifier.uri http://standard-demo.gcris.com/handle/123456789/5597
dc.language.iso en en_US
dc.publisher Izmir Institute of Technology en
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject.lcsh Tumor suppressor proteins en
dc.subject.lcsh p53 protein en
dc.subject.lcsh p53 antioncogene en
dc.title Regulation of human p53 tumor suppressor gene activity by thiol-depentdet oxidoreductases en_US
dc.type Master Thesis en_US
dspace.entity.type Publication
gdc.author.institutional Bekki, Gözde
gdc.description.department Food Engineering en_US
gdc.description.publicationcategory Tez en_US
gdc.oaire.accepatencedate 2011-01-01
gdc.oaire.diamondjournal false
gdc.oaire.impulse 0
gdc.oaire.influence 2.9837197E-9
gdc.oaire.influencealt 0
gdc.oaire.isgreen true
gdc.oaire.keywords Biyokimya
gdc.oaire.keywords Genetics
gdc.oaire.keywords Genetik
gdc.oaire.keywords Biochemistry
gdc.oaire.keywords Biology
gdc.oaire.keywords Biyoloji
gdc.oaire.popularity 7.325455E-10
gdc.oaire.popularityalt 0.0
gdc.oaire.publicfunded false

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