Determination of genetic diversity in national olive collection (Olea europaea L.) using SSR and SRAP markers and development of SNP marker for traceability of Memecik olive cultivar

Abstract

In this study genetic diversity of the Turkish olive collection was successfully characterized by using 13 SSR and 12 SRAP markers. For SSR marker analysis, we also added 3 European accessions to the national cultivars and binominal microsatellite data were detected by Qiaxcel software and clustered by NTSYS program. The UPGMA dendrogram showed good fit with the distance matrix (r . 0.85). While the outgroups were clustered with 0.27 similarity to national accessions, Turkish accessions clustered with 0.55 minimum similarity. Using SRAP markers we found 0.66 minimum similarity among Turkish accessions with a good fit between the distance data and UPGMA dendrogram (r . 0.83). The SSR and SRAP distance matrices were compared and they were found to correlated at r .0.23. To support the tree, ordination tests (PCA) for each marker system were performed and similar clustering was seen as in the trees. The other aim of this research was to develop SNP markers for traceability of Memecik accessions. We sequenced the anthocyanidin synthase gene of 11 exported olive accessions and aligned using Biolign program. There was no unique SNP that could be used to separate Memecik from the other accessions so we used a combination of SNPs. By using two probes at the same time, Memecik accession was distinguished from the other 10 exported accessions. In addition to find the SNP marker to trace Memecik accession, we developed the SNP marker to trace Trabzon Yağlık accessions.