An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue
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Date
2018
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Journal ISSN
Volume Title
Publisher
Mashhad Univ Med Sciences
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Abstract
Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.
Description
Kozanoglu, Ilknur/0000-0002-5268-1210; Cakmakoglu, Bedia/0000-0001-7960-9131; Bireller, Sinem/0000-0003-3465-1808; Ertugrul, Baris/0000-0003-3878-1829; Baran, Yusuf/0000-0002-1056-4673
Keywords
Apoptosis, Colon cancer, Stem cells, Cell death
Turkish CoHE Thesis Center URL
Fields of Science
Citation
8
WoS Q
Q3
Scopus Q
Q3

OpenCitations Citation Count
5
Source
Volume
21
Issue
5
Start Page
465
End Page
468
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Citations
Scopus : 9
PubMed : 5
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Mendeley Readers : 8